Coding

Part:BBa_K4665004:Design

Designed by: Fien Eickmans   Group: iGEM23_MSP-Maastricht   (2023-09-29)


SazCA membrane fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part results in a membrane fusion protein, meaning that extraction protocols for the protein should differ from normal extraction protocols


Source

SazCA is derived from Sulfurihydrogenibium azorense and INPN is derived from Pseudomonas syringae. Both sequences have been codon optimized for E. coli (BL21 DE3).

References